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plvx puro  (TaKaRa)


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    Structured Review

    TaKaRa plvx puro
    Plvx Puro, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plvx+puro/Puromycin/bio_rxiv__64898__2026__04__22__720056-320-24-25
    Average 96 stars, based on 3506 article reviews
    plvx puro - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Highly networked immunogen composition
    Article Snippet: .. Non-limiting examples of lentiviral vectors include pLVX-EF1alpha-AcGFP1-C1 (Clontech Catalog #631984), pLVX-EF1alpha-IRES-mCherry (Clontech Catalog #631987), pLVX-Puro (Clontech Catalog #632159), pLVX-IRES-Puro (Clontech Catalog #632186), pLenti6/V5-DESTTM (Thermo Fisher), pLenti6.2/V5-DESTTM (Thermo Fisher), pLKO.1 (Plasmid #10878 at Addgene), pLKO.3G (Plasmid #14748 at Addgene), pSico (Plasmid #11578 at Addgene), pLJM1-EGFP (Plasmid #19319 at Addgene), FUGW (Plasmid #14883 at Addgene), pLVTHM (Plasmid #12247 at Addgene), pLVUT-tTR-KRAB (Plasmid #11651 at Addgene), pLL3.7 (Plasmid #11795 at Addgene), pLB (Plasmid #11619 at Addgene), pWPXL (Plasmid #12257 at Addgene), pWPI (Plasmid #12254 at Addgene), EF.CMV.RFP (Plasmid #17619 at Addgene), pLenti CMV Puro DEST (Plasmid #17452 at Addgene), pLenti-puro (Plasmid #39481 at Addgene), pULTRA (Plasmid #24129 at Addgene), pLX301 (Plasmid #25895 at Addgene), pHIV-EGFP (Plasmid #21373 at Addgene), pLV-mCherry (Plasmid #36084 at Addgene), pLionII (Plasmid #1730 at Addgene), pInducer10-mir-RUP-PheS (Plasmid #44011 at Addgene). ..

    Article Title: Dolichol biosynthesis in yeast traces the expanded reaction pathway of higher eukaryotes
    Article Snippet: The quantification was performed by Agilent MassHunter Quantitative Analysis software (Agilent Technologies, CA, USA). .. Recombinant lentiviruses were produced in HEK293T cells as previously described using the vectors pUB81 TDA5. pUB81 is a lentiviral expression vector based on the pLVX-PURO (Clontech) plasmid containing EF-1α promoter. ..

    Article Title: A hepatitis B virus RNA-sensing and RNA-editing-dependent reporter system
    Article Snippet: .. To generate HepG2-NTCP-C4 stably expressing HBV-RADARS reporter, the reporter segment of HBV-RADARS plasmid was PCR amplified using primers ATCGGAATTCTAGCGTTTAAACTTAAGCTT and TATGGCTGATTATGATCTCTAGTCAGGTTTAAACGGGCCCTCTAG , followed by EcoRI and XbaI restriction digestion before ligating into the pLVX-Puro (Takara) lentiviral vector by T4 DNA ligase (Takara). .. Pseudotyped lentiviruses were packaged by Lenti-X Packaging Single Shots (VSV-G) (Takara) in Lenti-X 293T cells (Takara).

    Amplification:

    Article Title: Mechanisms of resistance to VHL loss-induced genetic and pharmacological vulnerabilities
    Article Snippet: .. HIF1A cDNA was amplified from HA-Clover-HIF-1alpha Wild-type (Addgene#163365). pLVX-puro (632164, Clonetech) was used to exogenously express the cDNA constructs. pLVX-HRE-ODD-GFP-hygro was generated by fusing GFP with HIF1A-ODD domain and cloned under a hypoxia response element modified minimal CMV promoter. ..

    Article Title: A hepatitis B virus RNA-sensing and RNA-editing-dependent reporter system
    Article Snippet: .. To generate HepG2-NTCP-C4 stably expressing HBV-RADARS reporter, the reporter segment of HBV-RADARS plasmid was PCR amplified using primers ATCGGAATTCTAGCGTTTAAACTTAAGCTT and TATGGCTGATTATGATCTCTAGTCAGGTTTAAACGGGCCCTCTAG , followed by EcoRI and XbaI restriction digestion before ligating into the pLVX-Puro (Takara) lentiviral vector by T4 DNA ligase (Takara). .. Pseudotyped lentiviruses were packaged by Lenti-X Packaging Single Shots (VSV-G) (Takara) in Lenti-X 293T cells (Takara).

    Article Title: A hepatitis B virus RNA-sensing and RNA-editing-dependent reporter system
    Article Snippet: .. Plasmids were constructed by PCR amplification of the HBV-RADARS backbone with primers F4 and R4, insertion of eGFP synthesized from IDT , or insertion of Puro r that is PCR amplified from pLVX-Puro (Takara #632164) using primer F5 and R5 ( Table S1 ). ..

    Construct:

    Article Title: Mechanisms of resistance to VHL loss-induced genetic and pharmacological vulnerabilities
    Article Snippet: .. HIF1A cDNA was amplified from HA-Clover-HIF-1alpha Wild-type (Addgene#163365). pLVX-puro (632164, Clonetech) was used to exogenously express the cDNA constructs. pLVX-HRE-ODD-GFP-hygro was generated by fusing GFP with HIF1A-ODD domain and cloned under a hypoxia response element modified minimal CMV promoter. ..

    Article Title: MicroRNA-124-3p suppresses lung cancer by targeting ITGB1/PI3K/p-AKT signal transduction pathway.
    Article Snippet: Worldwide, lung cancer is a leading cause of cancer-related death, and non-small cell lung cancer (NSCLC) represents the most common histological subtype.. Numerous studies have demonstrated that microRNAs (miRNAs) play critical roles in NSCLC pathogenesis. microRNA-124–3p (miR-124–3p) has been identified as a tumor-associated miRNA, and we confirmed its downregulation in NSCLC cells.. Functional assays showed that overexpression of miR-124–3p suppresses proliferation and migration of NSCLC cells, whereas its knockdown promotes these malignant phenotypes.

    Article Title: A hepatitis B virus RNA-sensing and RNA-editing-dependent reporter system
    Article Snippet: .. Plasmids were constructed by PCR amplification of the HBV-RADARS backbone with primers F4 and R4, insertion of eGFP synthesized from IDT , or insertion of Puro r that is PCR amplified from pLVX-Puro (Takara #632164) using primer F5 and R5 ( Table S1 ). ..

    Generated:

    Article Title: Mechanisms of resistance to VHL loss-induced genetic and pharmacological vulnerabilities
    Article Snippet: .. HIF1A cDNA was amplified from HA-Clover-HIF-1alpha Wild-type (Addgene#163365). pLVX-puro (632164, Clonetech) was used to exogenously express the cDNA constructs. pLVX-HRE-ODD-GFP-hygro was generated by fusing GFP with HIF1A-ODD domain and cloned under a hypoxia response element modified minimal CMV promoter. ..

    Clone Assay:

    Article Title: Mechanisms of resistance to VHL loss-induced genetic and pharmacological vulnerabilities
    Article Snippet: .. HIF1A cDNA was amplified from HA-Clover-HIF-1alpha Wild-type (Addgene#163365). pLVX-puro (632164, Clonetech) was used to exogenously express the cDNA constructs. pLVX-HRE-ODD-GFP-hygro was generated by fusing GFP with HIF1A-ODD domain and cloned under a hypoxia response element modified minimal CMV promoter. ..

    Article Title: Ionic Regulation of Mechanosurveillance and Metastasis via the MRTFA/KCNMB1 Axis
    Article Snippet: For doxycycline-inducible cell line generation, pLVX-TetON-Advanced (rtTA) (Takara Bio, #632162) was used. .. To over express YAP (Addgene #33091) and its active mutant YAP 5SA (Addgene #33093) were cloned into pLVX-puro (Takara Bio Catalog No. #632164). .. AT-3 cells were cultured in DMEM (Fisher Scientific # MT10017CV) containing 10% (vol/vol) FBS (Thermo Fisher # 26140079), 1 mM sodium pyruvate (Fisher Scientific #11-360-070), 2 mM non-essential amino acids (Thermo Fisher #11140050), 15 mM HEPES (Fisher Scientific #15-630-080), and 100 μM B-mercaptoethanol (Sigma #ES-007-E).

    Modification:

    Article Title: Mechanisms of resistance to VHL loss-induced genetic and pharmacological vulnerabilities
    Article Snippet: .. HIF1A cDNA was amplified from HA-Clover-HIF-1alpha Wild-type (Addgene#163365). pLVX-puro (632164, Clonetech) was used to exogenously express the cDNA constructs. pLVX-HRE-ODD-GFP-hygro was generated by fusing GFP with HIF1A-ODD domain and cloned under a hypoxia response element modified minimal CMV promoter. ..

    Expressing:

    Article Title: MicroRNA-124-3p suppresses lung cancer by targeting ITGB1/PI3K/p-AKT signal transduction pathway.
    Article Snippet: Worldwide, lung cancer is a leading cause of cancer-related death, and non-small cell lung cancer (NSCLC) represents the most common histological subtype.. Numerous studies have demonstrated that microRNAs (miRNAs) play critical roles in NSCLC pathogenesis. microRNA-124–3p (miR-124–3p) has been identified as a tumor-associated miRNA, and we confirmed its downregulation in NSCLC cells.. Functional assays showed that overexpression of miR-124–3p suppresses proliferation and migration of NSCLC cells, whereas its knockdown promotes these malignant phenotypes.

    Article Title: Dolichol biosynthesis in yeast traces the expanded reaction pathway of higher eukaryotes
    Article Snippet: The quantification was performed by Agilent MassHunter Quantitative Analysis software (Agilent Technologies, CA, USA). .. Recombinant lentiviruses were produced in HEK293T cells as previously described using the vectors pUB81 TDA5. pUB81 is a lentiviral expression vector based on the pLVX-PURO (Clontech) plasmid containing EF-1α promoter. ..

    Article Title: A hepatitis B virus RNA-sensing and RNA-editing-dependent reporter system
    Article Snippet: .. To generate HepG2-NTCP-C4 stably expressing HBV-RADARS reporter, the reporter segment of HBV-RADARS plasmid was PCR amplified using primers ATCGGAATTCTAGCGTTTAAACTTAAGCTT and TATGGCTGATTATGATCTCTAGTCAGGTTTAAACGGGCCCTCTAG , followed by EcoRI and XbaI restriction digestion before ligating into the pLVX-Puro (Takara) lentiviral vector by T4 DNA ligase (Takara). .. Pseudotyped lentiviruses were packaged by Lenti-X Packaging Single Shots (VSV-G) (Takara) in Lenti-X 293T cells (Takara).

    Cloning:

    Article Title: MicroRNA-124-3p suppresses lung cancer by targeting ITGB1/PI3K/p-AKT signal transduction pathway.
    Article Snippet: Worldwide, lung cancer is a leading cause of cancer-related death, and non-small cell lung cancer (NSCLC) represents the most common histological subtype.. Numerous studies have demonstrated that microRNAs (miRNAs) play critical roles in NSCLC pathogenesis. microRNA-124–3p (miR-124–3p) has been identified as a tumor-associated miRNA, and we confirmed its downregulation in NSCLC cells.. Functional assays showed that overexpression of miR-124–3p suppresses proliferation and migration of NSCLC cells, whereas its knockdown promotes these malignant phenotypes.

    Sequencing:

    Article Title: MicroRNA-124-3p suppresses lung cancer by targeting ITGB1/PI3K/p-AKT signal transduction pathway.
    Article Snippet: Worldwide, lung cancer is a leading cause of cancer-related death, and non-small cell lung cancer (NSCLC) represents the most common histological subtype.. Numerous studies have demonstrated that microRNAs (miRNAs) play critical roles in NSCLC pathogenesis. microRNA-124–3p (miR-124–3p) has been identified as a tumor-associated miRNA, and we confirmed its downregulation in NSCLC cells.. Functional assays showed that overexpression of miR-124–3p suppresses proliferation and migration of NSCLC cells, whereas its knockdown promotes these malignant phenotypes.

    Recombinant:

    Article Title: Dolichol biosynthesis in yeast traces the expanded reaction pathway of higher eukaryotes
    Article Snippet: The quantification was performed by Agilent MassHunter Quantitative Analysis software (Agilent Technologies, CA, USA). .. Recombinant lentiviruses were produced in HEK293T cells as previously described using the vectors pUB81 TDA5. pUB81 is a lentiviral expression vector based on the pLVX-PURO (Clontech) plasmid containing EF-1α promoter. ..

    Article Title: Dominant spinal muscular atrophy linked mutations in the cargo binding domain of BICD2 result in altered interactomes and dynein hyperactivity
    Article Snippet: Recombinant DNA reagent , pEGFP-C3 , Clontech , Discontinued by manufacturer , . .. Recombinant DNA reagent , pLVX-Puro , Takara , 631849 , . .. Recombinant DNA reagent , BICD2_wt-mNeon in pLVX-Puro , This paper , Available upon request , Materials and methods.

    Produced:

    Article Title: Dolichol biosynthesis in yeast traces the expanded reaction pathway of higher eukaryotes
    Article Snippet: The quantification was performed by Agilent MassHunter Quantitative Analysis software (Agilent Technologies, CA, USA). .. Recombinant lentiviruses were produced in HEK293T cells as previously described using the vectors pUB81 TDA5. pUB81 is a lentiviral expression vector based on the pLVX-PURO (Clontech) plasmid containing EF-1α promoter. ..

    Stable Transfection:

    Article Title: A hepatitis B virus RNA-sensing and RNA-editing-dependent reporter system
    Article Snippet: .. To generate HepG2-NTCP-C4 stably expressing HBV-RADARS reporter, the reporter segment of HBV-RADARS plasmid was PCR amplified using primers ATCGGAATTCTAGCGTTTAAACTTAAGCTT and TATGGCTGATTATGATCTCTAGTCAGGTTTAAACGGGCCCTCTAG , followed by EcoRI and XbaI restriction digestion before ligating into the pLVX-Puro (Takara) lentiviral vector by T4 DNA ligase (Takara). .. Pseudotyped lentiviruses were packaged by Lenti-X Packaging Single Shots (VSV-G) (Takara) in Lenti-X 293T cells (Takara).

    Polymerase Chain Reaction:

    Article Title: A hepatitis B virus RNA-sensing and RNA-editing-dependent reporter system
    Article Snippet: .. To generate HepG2-NTCP-C4 stably expressing HBV-RADARS reporter, the reporter segment of HBV-RADARS plasmid was PCR amplified using primers ATCGGAATTCTAGCGTTTAAACTTAAGCTT and TATGGCTGATTATGATCTCTAGTCAGGTTTAAACGGGCCCTCTAG , followed by EcoRI and XbaI restriction digestion before ligating into the pLVX-Puro (Takara) lentiviral vector by T4 DNA ligase (Takara). .. Pseudotyped lentiviruses were packaged by Lenti-X Packaging Single Shots (VSV-G) (Takara) in Lenti-X 293T cells (Takara).

    Article Title: A hepatitis B virus RNA-sensing and RNA-editing-dependent reporter system
    Article Snippet: .. Plasmids were constructed by PCR amplification of the HBV-RADARS backbone with primers F4 and R4, insertion of eGFP synthesized from IDT , or insertion of Puro r that is PCR amplified from pLVX-Puro (Takara #632164) using primer F5 and R5 ( Table S1 ). ..

    Synthesized:

    Article Title: A hepatitis B virus RNA-sensing and RNA-editing-dependent reporter system
    Article Snippet: .. Plasmids were constructed by PCR amplification of the HBV-RADARS backbone with primers F4 and R4, insertion of eGFP synthesized from IDT , or insertion of Puro r that is PCR amplified from pLVX-Puro (Takara #632164) using primer F5 and R5 ( Table S1 ). ..

    Mutagenesis:

    Article Title: Ionic Regulation of Mechanosurveillance and Metastasis via the MRTFA/KCNMB1 Axis
    Article Snippet: For doxycycline-inducible cell line generation, pLVX-TetON-Advanced (rtTA) (Takara Bio, #632162) was used. .. To over express YAP (Addgene #33091) and its active mutant YAP 5SA (Addgene #33093) were cloned into pLVX-puro (Takara Bio Catalog No. #632164). .. AT-3 cells were cultured in DMEM (Fisher Scientific # MT10017CV) containing 10% (vol/vol) FBS (Thermo Fisher # 26140079), 1 mM sodium pyruvate (Fisher Scientific #11-360-070), 2 mM non-essential amino acids (Thermo Fisher #11140050), 15 mM HEPES (Fisher Scientific #15-630-080), and 100 μM B-mercaptoethanol (Sigma #ES-007-E).



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